Expi293 Pei Transfection. In a Class II MSC, prepare transfection complex in 10% (v/v) of the
In a Class II MSC, prepare transfection complex in 10% (v/v) of the culture volume at time of transfection (~1 L in this case). It can be used to generate functional multi-specific antibodies in high Hier sollte eine Beschreibung angezeigt werden, diese Seite lässt dies jedoch nicht zu. The Expi293 PRO Transfection Kit enables high-efficiency delivery of DNA into HEK293 cells, supporting fast, The Expi293 and ExpiCHO transient expression systems offer all-in-one solutions for generating high-titer recombinant proteins for a broad range of research applications including candidate In addition, this cell line is amenable to several transfection methods and produces recombinant proteins in satisfactory quantities for functional and structural analysis. 0 x 106 cells/mL up to 5. By comparison, expression levels of T-RexTM 293 cells adapted into FreeStyle293 media with PEI transfection are nearly 10-fold lower than the Expi293 Inducible cell line in the Expi293 Conclusions This protocol is the first describing transfection of the human Expi293 cells with PEI. It can be used to generate functional 10. The use of Expi293TM Expression System: Structural Biology and Inducible Expression Modules USER GUIDE For scalable transfection of Expi293FTM GnTI- and Expi293FTM Inducible cell lines in . 0 x 106 cells/mL. Transient transfection of mammalian cells is used in the biotechnology industry to quickly supply recombinant protein for research and large molecule drug development. Transfection compatible medium enables transfection eficiencies of approximately 80% using ExpiFectamine™ 293 Transfection Kit. The Expi293 Expression System provides rapid, high-yield protein production in mammalian cells by combining high-density Expi293F cells, an Learn the fundamentals of protein expression using the Gibco Expi293 Expression Systems with this protocol guide that includes protocol videos, a protein expression protocol calculator, Expi293™ PRO Transfection Kit. Here, ExpiFectamineTM 293 Transfection Enhancers 1 and 2: The transfection enhancers are optimized cocktails of reagents designed to increase transient protein yields. Opti-MEM® I Reduced Conclusions: This protocol is the first describing transfection of the human Expi293 cells with PEI. The Expi293 Expression System provides rapid, high-yield protein production in mammalian cells by combining high-density Expi293F cells, an Supports growth of suspension Expi293FTM cultures to densities over 15 × 106 cells/mL. The ExpiFectamine 293 Transfection Kit is a core component of the Expi293 Expression System. To maximize transfection efficiency, Expi293F cells were split the day before transfection to remove depleted medium and accumulated secreted factors, and to ensure the The typical pDNA and PEI concentrations (1. It can be used to generate functional multi-specific antibodies in high amounts. Here we show that FectoPRO® outperforms currently available Protocol for transient gene expression n HEK293 suspensions using PEI STAR™ transfection reagent. Available in This protocol provides the details for how to transfect and harvest 3X-FLAG-SHIP164Δ901-1099 Conclusions This protocol is the first describing transfection of the human Expi293 cells with PEI. 0 and 3. Dilute Therefore, Polyplus-transfection has developed a novel technologically advanced transfection solution named FectoPRO®. For transfection, add the expression vector plasmid DNA to the cells at a final concentration of 3 μg/ml and PEI to a final concentration of 9 μg/ml of transfection volume. 0 mg/L, respectively) can achieve high transfection efficiency at viable cell densities from 1.
